Hi Bruce,
You mentioned the aperture setting used with your 300 mm f/2.8 + 1.4x but not the distance between the camera and test target.
For technical reasons there will be a small variation of the AFFT required depending upon aperture and distance to the test target. Canon recommend the distance to the target should be about 50 x effective focal length = 50 x 420 mm in your case or about 21 m. Unless you have a big house, this is not easily achieved indoors and for this reason I do my tests outside where I am fortunate to have a long enough concrete deck. It makes a good optical bench and I can use sunlight to illuminate the test target..
The aperture used for AFFT is also important. If you have a sensitive AFFT setup you will be able to clearly see a difference between the AFFT required at full aperture and when closed down a stop. For this reason, I usually do my adjustment with the aperture closed down one stop which will be closer to the aperture I usually use in the field. I very rarely use any of my lenses at full aperture because the depth of field is usually too small to get all the bird in sharp focus. So for your 300 mm f/2.8 + 1.4x I would do the AFFT at aperture f/5. You will find there is very little variation of AFFT for f/5 - f/11 but there will be a measurable difference between f/4 and f/5.
There can also be a slight variation in AFFT depending upon the light source. This is usually a very small effect and provided you have enough light to use a reasonable ISO, say less than ISO 800, it will not be an issue. If you can test outside using direct sunlight, this will be the perfect light source. One general comment about outdoor testing; try to do the tests in the morning before your test range gets too warm. If you are testing over a warm 21 m surface you may find the AFFT results vary alarmingly; to avoid this problem it is best to do the testing in the morning. However, with the kind of weather we are currently experiencing in Victoria, it will make no difference whether you are testing in the morning or afternoon but in the summer it will usually only be possible to get consistent results during the morning.
Hope this helps, good luck,
Ian